Recombinant Mouse Gas6

Growth arrest-specific 6, also known as GAS6 is a growth factor¹ that serves as a ligand for members of the Axl subfamily of receptor protein-tyrosine kinases.² GAS6 is expressed in hematopoietic tissue.³ GAS6 function as a direct regulator of osteoclastic function; they give an insight into the role of these vitamin K-dependent ligands in bone resorption in vivo.² GAS6 has a potential therapeutic target to minimize graft-versus-host disease (GVHD).⁴

The biological activity of Mouse Gas6 was determined by its ability to bind rmAxl/Fc in a functional ELISA. Immobilized rmAxl/Fc at 2 μg/ml (100 μl/well) can bind rmGas6 with a linear range of 0.08 - 5 ng/ml.

Histidine Tag

dqctpnpcdk kgthicqdlm gnffcvctdg wggrlcdkdv necvqknggc sqvchnkpgs fqcachsgfs lasdgqtcqd idectdsdtc gdarcknlpg sysclcdegy tysskektcq dvdecqqdrc eqtcvnspgs ytchcdgrgg lklspdmdtc edilpcvpfs maksvkslyl grmfsgtpvi rlrfkrlqpt rllaefdfrt fdpegvlffa ggrsdstwiv lglragrlel qlryngvgri tssgptinhg mwqtisveel ernlvikvnk davmkiavag elfqlergly hlnltvggip fkeselvqpi nprldgcmrs wnwlngedsa iqetvkantk mqcfsvterg sffpgngfat yrlnytrtsl dvgtettwev kvvarirpat dtgvllalvg dddvvisval vdyhstkklk kqlvvlaved valalmeikv cdsqehtvtv slregeatle vdgtkgqsev staqlqerld tlkthlqgsv htyvgglpev svisapvtaf yrgcmtlevn gkildldtas ykhsditshs cppvehatph hhhhh

Asp115

Lyophilized

The predicted molecular weight of Recombinant Mouse Gas6 is Mr 62 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 72 kDa (reducing conditions).

62

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Suda, T. et al. (1998) Stem Cells 16: 229
2. Schneider, C. et al. (2001) Mol Cell Biol. 21: 902
3. Aglietta, M. et al. (1997) Exp Hematol. 25: 1219
4. Angelillo-Scherrer, A. et al. (2005) J Clin Invest. 115(2):237-46.