Anti-Mouse Gas6 (Clone 126618)

Anti-Mouse Gas6 (Clone 126618)

Product No.: G680

[product_table name="All Top" skus="G680"]

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Clone
126618
Target
Gas6
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
AXLLG, AXSF, DKFZp666G247, FLJ34709, Growth Arrest Specific 6
Isotype
IgG2a
Applications
ELISA Indirect
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse Gas6
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:R380. The detection sensitivity of this indirect ELISA for Mouse Gas6 is approximately 1 ng/well.
Western Blotting: To detect Mouse Gas6 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Gas6 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Growth Arrest Specific Protein 6 (Gas6) (Clone 126618) recognizes an epitope on Mouse Gas6. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Growth arrest-specific 6, also known as GAS6 is a growth factor1 that serves as a ligand for members of the Axl subfamily of receptor protein-tyrosine kinases.2 GAS6 is expressed in hematopoietic tissue.3 GAS6 function as a direct regulator of osteoclastic function; they give an insight into the role of these vitamin K-dependent ligands in bone resorption in vivo.2 GAS6 has a potential therapeutic target to minimize graft-versus-host disease (GVHD).4

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Suda, T. et al. (1998) Stem Cells 16: 229
2. Schneider, C. et al. (2001) Mol Cell Biol. 21: 902
3. Aglietta, M. et al. (1997) Exp Hematol. 25: 1219
4. Angelillo-Scherrer, A. et al. (2005) J Clin Invest. 115(2):237-46.
Indirect Elisa Protocol
General Western Blot Protocol
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Formats Available

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Prod No.
Description
G680
Products are for research use only. Not for use in diagnostic or therapeutic procedures.