Anti-Mouse Gas6 – Biotin
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse Gas6 (Accession # CAA42507) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830248 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse Gas6 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Gas6 is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G655. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:G659 as the capture antibody at 0.2-0.8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse Gas6. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Growth Arrest-Specific Gene 6 (Gas6) recognizes Mouse Gas6. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Growth arrest-specific 6, also known as GAS6 is a growth factor1 that serves as a ligand for members of the Axl subfamily of receptor protein-tyrosine kinases.2 GAS6 is expressed in hematopoietic tissue.3 GAS6 function as a direct regulator of osteoclastic function; they give an insight into the role of these vitamin K-dependent ligands in bone resorption in vivo.2 GAS6 has a potential therapeutic target to minimize graft-versus-host disease (GVHD).4 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Suda, T. et al. (1998) Stem Cells 16: 229 2. Schneider, C. et al. (2001) Mol Cell Biol. 21: 902 3. Aglietta, M. et al. (1997) Exp Hematol. 25: 1219 4. Angelillo-Scherrer, A. et al. (2005) J Clin Invest. 115(2):237-46. Technical ProtocolsELISA Det  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
