Recombinant Mouse ICAM-1

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Pricing & Details

Product. No.I-587
Alternate Names
CD54, Ly-47, MALA-2, Intercellular adhesion molecule 1, P3.58, BB2
Expression Host
NS0 cells
Species
Mouse
Prod No.
Size
Price
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I-587-50 µg
50 µg
$285.00
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Background

ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse ICAM-1 was determined by the ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to rmICAM coated plates (12.5 μg/ml with 100 μl/well), approximately 65 - 95% will adhere after PMA stimulation for 1 hour at RT.
Fusion Protein Tag
Fc Fusion Protein
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
qvs ihpreaflpq ggsvqvncss sckedlslgl etqwlkdele sgpnwklfel seigedsspl cfencgtvqs sasatitvys fpesvelrpl pawqqvgkdl tlrchvdgga prtqlsavll rgeeilsrqp vgghpkdpke itftvlasrg dhganfscrt eldlrpqgla lfsnvsears lrtfdlpati pkldtpdlle vgtqqklfcs leglfpasea riylelggqm ptqestnssd svsatalvev teefdrtlpl rcvleladqi letqrtltvy nfsapvltls qlevsegsqv tvkceahsgs kvvllsgvep rpptpqvqft lnassedhkr sffcsaalev agkflfknqt lelhvlygpr ldetdclgnw twqegsqqtl kcqawgnpsp kmtcrrkadg allpigvvks vkqemngtyv chafsshgnv trnvyltvly hsqnniegrm dpkscdktht cppcpapell ggpsvflfpp kpkdtlmisr tpevtcvvvd vshedpevkf nwyvdgvevh naktkpreeq ynstyrvvsv ltvlhqdwln gkeykckvsn kalpapiekt iskakgqpre pqvytlppsr deltknqvsl tclvkgfyps diavewesng qpennykttp pvldsdgsff lyskltvdks rwqqgnvfsc svmhealhnh ytqkslslsp g
N-terminal Sequence Analysis
Gln28
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse ICAM-1 is Mr 76.8 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 110-130 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives present.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Sriramarao, P. et al. (2018) Am J Physiol Lung Cell Mol Physiol. 315(2):L227-L240. PubMed
2. Li, S. et al. (2009) Biochem Biophys Res Commun. 381: 459
3. Wolf, S. et al. (2009) Pharmacol Rep 61: 22
4. Ozcan, U. et al. (2009) Arch Gynecol Obstet.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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