Recombinant Human ICAM-1

ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

The biological activity of Human ICAM-1 was determined by the ability of the immobilized protein to support the adhesion of HSB2 cells, a human peripheral blood acute lymphoblastic leukemia cell line. When 5 x 10⁴ cells/well are added to recombinant human ICAM/Fc coated plates (12.5 μg/ml with 100 μl/well), approximately 60 - 70% will adhere after PMA stimulation for 1 hour at RT.

Fc Fusion Protein

qts vspskvilpr ggsvlvtcst scdqpkllgi etplpkkell lpgnnrkvye lsnvqedsqp mcysncpdgq staktfltvy wtpervelap lpswqpvgkn ltlrcqvegg apranltvvl lrgekelkre pavgepaevt ttvlvrrdhh ganfscrtel dlrpqglelf entsapyqlq tfvlpatppq lvsprvlevd tqgtvvcsld glfpvseaqv hlalgdqrln ptvtygndsf sakasvsvta edegtqrltc avilgnqsqe tlqtvtiysf papnviltkp evsegtevtv kceahprakv tlngvpaqpl gpraqlllka tpedngrsfs csatlevagq lihknqtrel rvlygprlde rdcpgnwtwp ensqqtpmcq awgnplpelk clkdgtfplp igesvtvtrd legtylcrar stqgevtrev tvnvlsprye iegrmdpksc dkthtcppcp apellggpsv flfppkpkdt lmisrtpevt cvvvdvshed pevkfnwyvd gvevhnaktk preeqynsty rvvsvltvlh qdwlngkeyk ckvsnkalpa piektiskak gqprepqvyt lppsrdeltk nqvsltclvk gfypsdiave wesngqpenn ykttppvlds dgsfflyskl tvdksrwqqg nvfscsvmhe alhnhytqks lslspgk

No results obtained: Gln28 predicted

Lyophilized

The predicted molecular weight of Recombinant Human ICAM-1 is Mr 76 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 110-125 kDa.

76

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Li, S. et al. (2009) Biochem Biophys Res Commun. 381: 459
2. Wolf, S. et al. (2009) Pharmacol Rep 61: 22
3. Ozcan, U. et al. (2009) Arch Gynecol Obstet.