Anti-Rat ICAM-1 – Biotin

Anti-Rat ICAM-1 – Biotin

Product No.: I-786

[product_table name="All Top" skus="I-786"]

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Target
ICAM-1
Product Type
Polyclonal Antibody
Alternate Names
CD54, Ly-47, MALA-2, Intercellular adhesion molecule 1, cell surface glycoprotein P3.58, BB2
Applications
ELISA Det
,
WB

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Product Size

Data

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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Goat
Immunogen
NS0-Derived Recombinant Rat ICAM-1 Extracellular Domain (Accession # Q00238)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Rat ICAM-1 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Rat ICAM-1 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL. Use in combination with: Capture Reagent: Rat ICAM‑1/CD54 Antibody. Standard: Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Rat Intercellular Adhesion Molecule-1 (ICAM-1) recognizes Rat ICAM-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Antigen Distribution
CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes.
Background
ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Li, S. et al. (2009) Biochem Biophys Res Commun. 381: 459
2. Wolf, S. et al. (2009) Pharmacol Rep 61: 22
3. Ozcan, U. et al. (2009) Arch Gynecol Obstet.
ELISA Det
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.