Anti-Human Intra-Cellular Adhesion Molecule 3 (ICAM-3)

Anti-Human Intra-Cellular Adhesion Molecule 3 (ICAM-3)

Product No.: I-733

[product_table name="All Top" skus="I-733"]

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Target
ICAM-3
Product Type
Polyclonal Antibody
Alternate Names
Intra-Cellular Adhesion Molecule 3, CD50, CDW50, ICAM-R
Applications
ELISA Indirect
,
FC
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Sheep
Immunogen
CHO Cell-Derived Recombinant Human ICAM-3 Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human ICAM-3 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as and a chromogenic substrate such as PN:T343. The detection limit for rhICAM-3 is approximately 2 ng/lane and 20 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells.
Additional Applications Reported In Literature ?
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Sheep Anti-Human Intracellular Adhesion Molecule 3 (ICAM-3) recognizes Human ICAM-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50) is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins that bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signaling molecule.
ICAM-3 is potentially the most important ligand for LFA-1 in the initiation of the immune response because the expression of ICAM-1 on resting leukocytes is low.1 ICAM-3 is closely related to ICAM-1. 1 ICAM-3 is essentially restricted to the haemopoietic system and is reciprocal in its expression to ICAM-1, in that it is present on resting cells and its level falls as a result of cell activation.2

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Simmons, DL. et al. (1992) Nature 360: 481
2. Mason, DY. et al. (1994) J Clin Pathol 47: 143
Indirect Elisa Protocol
Flow Cytometry
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.