Anti-Glutathione-S-Transferase (GST) Antibody (18842)
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Antibody DetailsProduct DetailsHost Species Goat Immunogen Glutathione-S-Transferase (GST) from Schistosoma japonicum. Product Concentration 1.0 mg/ml Formulation PBS, pH 7.2, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by GST affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000-1:20,000.
Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Polyclonal Antibody specific to Glutathione-S-Transferase (GST). Background A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. Antigen DetailsResearch Area Epitope Tags References & CitationsTechnical Protocols |
