Anti-Glutathione-S-Transferase (GST) Antibody (18842)

Anti-Glutathione-S-Transferase (GST) Antibody (18842)

Product No.: 18842

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Target
Glutathione-S-Transferase (GST)
Product Type
Polyclonal
Applications
ELISA
,
ICC
,
IP
,
WB

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Select Product Size
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Antibody Details

Product Details

Host Species
Goat
Immunogen
Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Product Concentration
1.0 mg/ml
Formulation
PBS, pH 7.2, 0.09% sodium azide.
State of Matter
Liquid
Product Preparation
Purified by GST affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at 2-8°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at a dilution of 1:1,000-1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use 1-4ug/mg lysate

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500.

These are recommended dilutions.

End users should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Polyclonal Antibody specific to Glutathione-S-Transferase (GST).
Background
A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

Antigen Details

Research Area
Epitope Tags

References & Citations

Indirect Elisa Protocol
ICC
Immunoprecipitation Protocol
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.