Anti-Glutathione-S-Transferase (GST) Antibody (18844)

Anti-Glutathione-S-Transferase (GST) Antibody (18844)

Product No.: 18844

- -
- -
Target
Glutathione-S-Transferase (GST)
Product Type
Polyclonal
Alternate Names
GST 28
Isotype
Whole IgG
Applications
ELISA
,
ICC
,
IP
,
WB

- -
- -
Select Product Size

Data

<b>Detection of GST-tagged Protein by western blot.</b> <i>Samples:</i> 0.1Detection of GST-tagged Protein by western blot. Samples: 0.1, 0.3, or 1.0.µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity purified, rabbit anti-GST antibody 18844 used for WB at 0.06 µg/ml (1:15,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
- -
- -

Antibody Details

Product Details

Host Species
Rabbit
Immunogen
Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Product Concentration
1.0 mg/ml
Formulation
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
State of Matter
Liquid
Product Preparation
Purified by affinity on GST
Storage and Handling
This antibody is stable for at least one (1) year at 2-8°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at a dilution of 1:1,000- 1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use 1-4ug/mg lysate.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to Glutathione-S-transferase (GST). Antibody was affinity-purified on GST.
Background
A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

Antigen Details

Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
UniProt.org
Research Area
Epitope Tags

References & Citations

Indirect Elisa Protocol
ICC
Immunoprecipitation Protocol
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.