Anti-T7 Antibody (18864)

Anti-T7 Antibody (18864)

Product No.: 18864

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Target
T7
Product Type
Polyclonal
Isotype
Whole IgG
Applications
ELISA
,
ICC
,
IP
,
WB

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Select Product Size

Data

<b>Detection of T7-tagged Protein by western blot.</b> <i>Samples:</i> 200Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-T7 antibody 18864 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
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Antibody Details

Product Details

Host Species
Rabbit
Immunogen
T7 (MASMTGGQQMG) conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
State of Matter
Liquid
Product Preparation
Purified by peptide immuno-affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at 2-8°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at a dilution of 1:1,000- 1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use 1-4ug/mg lysate.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to T7. Antibody was affinity-purified on the peptide.
Background
The T7 tag is an 11 amino acid peptide (MASMTGGQQMG) derived from the leader sequence of T7 bacteriophage gene10 which encodes a T7 major capsid protein. The T7 tag serves as a tag in many expression vectors, and antibodies specific for the T7 tag are useful for identifying and isolating T7-tagged proteins.

Antigen Details

Research Area
Epitope Tags

References & Citations

Indirect Elisa Protocol
ICC
Immunoprecipitation Protocol
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.