Recombinant Mouse IL-10

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Pricing & Details

Product. No.I-211
Alternate Names
Interleukin-10, CSIF, IL10A, MGC126450, MGC126451, TGIF
Expression Host
E. coli
Species
Mouse
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-211-5 µg
5 µg
$249.00
In stock
Max:
Min: 1
Step: 1
I-211-25 µg
25 µg
$819.00
In stock
Max:
Min: 1
Step: 1
I-211-100 µg
100 µg
$1,369.00
In stock
Max:
Min: 1
Step: 1

Background

IL-10 is a homodimeric, anti-inflammatory cytokine of 17-21 kD with various, pleiotropic, effects in immunoregulation and inflammation. It increases antibody production, in addition to enhancing B cell survival and proliferation. IL-10 inhibits both the synthesis of pro-inflammatory cytokines and the actions of Natural Killer (NK) cells during the immune response to viral infection. Moreover, IL-10 is involved in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens. IL-10 can also block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. In mice, lack of IL-10 has been shown to cause inflammation and pain via COX activation resulting in vascular endothelial and cardiac dysfunctions. Additionally, IL-10 is linked to myokines, a form of cytokine produced in muscle cells that participates in tissue regeneration and repair, maintenance of healthy bodily functioning, and homeostasis in the immune system. Exercise is known to increase circulating levels of IL-10. Hence, it is thought that physical exercise promotes an environment of anti-inflammatory cytokines. Furthermore, knockout studies of IL-10 suggest this cytokine is crucial for counteracting the hyperactive immune response in the intestinal tract. It has been reported that treatment with recombinant IL-10 producing bacteria has been beneficial in patients with Crohn's disease.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
srgqysredn ncthfpvgqs hmllelrtaf sqvktffqtk dqldnilltd slmqdfkgyl gcqalsemiq fylvevmpqa ekhgpeikeh lnslgeklkt lrmrlrrchr flpcenkska veqvksdfnk lqdqgvykam nefdifinci eaymmikmks
N-terminal Sequence Analysis
Ser19
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IL-10 is Mr 18 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 17 kDa.
Formulation
This recombinant protein solution was 0.2 µm filtered and is supplied in a sterile solution containing 10mM Tris, 75 mM NaCl.
Storage and Stability
This recombinant protein solution is stable for six months when stored desiccated at 2°C to 8°C.
Country of Origin
USA
Shipping
Polar Packs
PubMed
NCBI Gene Bank

References & Citations

1. Akdis, CA. et al. (2009) Mol. Immunol. 46: 622
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.