Anti-Porcine IL-10

Pricing & Details

Product No.I-696
Clone
148801
Protein
IL-10
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CSIF, IL10A, MGC126450, MGC126451, TGIF, Interleukin-10
Isotype
Mouse
IgG2b
Applications
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-696-25 µg
25 µg
$190.00
In stock
Max:
Min: 1
Step: 1
I-696-500 µg
500 µg
$525.00
In stock
Max:
Min: 1
Step: 1
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Antibody Details

Product Details

Reactivity Species
Porcine
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Porcine Interleukin-10
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse IL-10 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IL-10 (at 40 ng/ml) is 0.05 - 0.15 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 148801 recognizes an epitope on mouse IL-10.
Background
IL-10 is a homodimeric, anti-inflammatory cytokine of 17-21 kD with various, pleiotropic, effects in immunoregulation and inflammation. It increases antibody production, in addition to enhancing B cell survival and proliferation. IL-10 inhibits both the synthesis of pro-inflammatory cytokines and the actions of NK cells during the immune response to viral infection. Moreover, IL-10 is involved in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens. IL-10 can also block NF-B activity, and is involved in the regulation of the JAK-STAT signaling pathway. In mice, lack of IL-10 has been shown to cause inflammation and pain via COX activation resulting in vascular endothelial and cardiac dysfunctions. Additionally, IL-10 is linked to myokines, a form of cytokine produced in muscle cells that participates in tissue regeneration and repair, maintenance of healthy bodily functioning, and homeostasis in the immune system. Exercise is known to increase circulating levels of IL-10. Hence, it is thought that physical exercise promotes an environment of anti-inflammatory cytokines. Furthermore, knockout studies of IL-10 suggest this cytokine is crucial for counteracting the hyperactive immune response in the intestinal tract. It has been reported that treatment with recombinant IL-10 producing bacteria has been beneficial in patients with Crohn's disease.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Akdis, CA. et al. (2009) Mol. Immunol. 46: 622
2. Medzhitov, R. et al. (2017) Science. 356(6337):513-519.

Formats Available

Prod No.
Description
I-696
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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