Recombinant Rat IL-10 (Cys 167 Tyr)

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Pricing & Details

Product. No.I-222
Alternate Names
Interleukin-10, CSIF, IL10A, MGC126450, MGC126451, TGIF
Expression Host
E. coli
Species
Rat
Prod No.
Size
Price
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Qty
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I-222-10 µg
10 µg
$309.00
In stock
Max:
Min: 1
Step: 1

Background

IL-10 is a homodimeric, anti-inflammatory cytokine of 17-21 kD with various, pleiotropic, effects in immunoregulation and inflammation. It increases antibody production, in addition to enhancing B cell survival and proliferation. IL-10 inhibits both the synthesis of pro-inflammatory cytokines and the actions of NK cells during the immune response to viral infection. Moreover, IL-10 is involved in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens. IL-10 can also block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. In mice, lack of IL-10 has been shown to cause inflammation and pain via COX activation resulting in vascular endothelial and cardiac dysfunctions. Additionally, IL-10 is linked to myokines, a form of cytokine produced in muscle cells that participates in tissue regeneration and repair, maintenance of healthy bodily functioning, and homeostasis in the immune system. Exercise is known to increase circulating levels of IL-10. Hence, it is thought that physical exercise promotes an environment of anti-inflammatory cytokines. Furthermore, knockout studies of IL-10 suggest this cytokine is crucial for counteracting the hyperactive immune response in the intestinal tract. It has been reported that treatment with recombinant IL-10 producing bacteria has been beneficial in patients with Crohn's disease.

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
skghsirgdn ncthfpvsqt hmlrelraaf sqvktffqkk dqldnilltd sllqdfkgyl gcqalsemik fylvevmpqa enhgpeikeh lnslgeklkt lwiqlrrchr flpcenkska veqvkndfnk lqdkgvykam nefdifinci eayvtlkmkn (Cys167Tyr)
N-terminal Sequence Analysis
Ser19
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Rat IL-10 is Mr 18.7 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in sodium phosphate (NaH2PO4) and sodium chloride (NaCl).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Akdis, CA. et al. (2009) Mol. Immunol. 46: 622
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.