Recombinant Mouse IL-27 (p28)

Interleukin-27 (IL-27) is a novel IL-12 family member that plays a role in the early regulation of T helper cell 1 initiation, including induction of T-bet and IL-12 receptor beta 2 expression.¹ IL-27 is produced by antigen-presenting cells² and plays an important function in regulating the activity of B and T lymphocytes.3 IL27 may be a potential therapeutic agent against rheumatoid arthritis (RA) at the onset of the disease.⁴

The biological activity of Mouse IL-27 was determined by dose-dependent inhibition of TGF-beta & IL-6-induced IL-17A expression in mouse CD4 splenocytes. 50 ng/ml of mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay.

MFPTDPLSLQ ELRREFTVSL YLARKLLSEV QGYVHSFAES RLPGVNLDLL PLGYHLPNVS LTFQAWHHLS DSERLCFLAT TLRPFPAMLG GLGTQGTWTS SEREQLWAMR LDLRDLHRHL RFQVLAAGFK CSKEEEDKEE EEEEEEEEKK LPLGALGGPN QVSSQVSWPQ LLYTYQLLHS LELVLSRAVR DLLLLSLPRR PGSAWDS

Lyophilized

The predicted molecular weight of Recombinant Mouse IL-27 is Mr 23.7 kDa.

23.7

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1. Yoshimoto, T. et al. (2004) Cancer Res. 64: 1152
2. De Sauvage, FJ. et al. (2003) Proceedings of the National Acad of Sci of USA 100: 15047
3. Devergne, O. et al. (2006) J Immunol. 176: 5890
4. Liew, FY. et al. (2008) Ann Rheum Dis. 67: 1474