Interleukin-27 (IL-27) is a novel IL-12 family member that plays a role in the early regulation of T helper cell 1 initiation, including induction of T-bet and IL-12 receptor beta 2 expression.1 IL-27 is produced by antigen-presenting cells2 and plays an important function in regulating the activity of B and T lymphocytes.3 IL27 may be a potential therapeutic agent against rheumatoid arthritis (RA) at the onset of the disease.4
>97% by SDS-PAGE and HPLC
<0.1 EU/µg as determined by the LAL method
The biological activity of Mouse IL-27 was determined by dose-dependent inhibition of TGF-beta & IL-6-induced IL-17A expression in mouse CD4 splenocytes. 50 ng/ml of mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay.
Protein Accession No.
Amino Acid Sequence
MFPTDPLSLQ ELRREFTVSL YLARKLLSEV QGYVHSFAES RLPGVNLDLL PLGYHLPNVS LTFQAWHHLS DSERLCFLAT TLRPFPAMLG GLGTQGTWTS SEREQLWAMR LDLRDLHRHL RFQVLAAGFK CSKEEEDKEE EEEEEEEEKK LPLGALGGPN QVSSQVSWPQ LLYTYQLLHS LELVLSRAVR DLLLLSLPRR PGSAWDS
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IL-27 is Mr 23.7 kDa.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Yoshimoto, T. et al. (2004) Cancer Res. 64: 1152
2. De Sauvage, FJ. et al. (2003) Proceedings of the National Acad of Sci of USA 100: 15047
3. Devergne, O. et al. (2006) J Immunol. 176: 5890
4. Liew, FY. et al. (2008) Ann Rheum Dis. 67: 1474
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.