Recombinant Mouse IL-6

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Pricing & Details

Product. No.I-208
Alternate Names
Interleukin-6, BSF2, HPGF, HSF, IFNB2, MGI-2, HGF, B Cell Differentiation Factor [BCDF]
Expression Host
E. coli
Species
Mouse
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-208-5 µg
5 µg
$239.00
In stock
Max:
Min: 1
Step: 1
I-208-25 µg
25 µg
$765.00
In stock
Max:
Min: 1
Step: 1
I-208-200 µg
200 µg
$1,399.00
In stock
Max:
Min: 1
Step: 1

Background

IL-6 is a pleotropic 26 kD protein that can act as both a pro-inflammatory cytokine and an anti-inflammatory myokine, a form of cytokine produced in muscle cells that participates in tissue regeneration and repair, maintenance of healthy bodily functioning, and homeostasis within the immune system. IL-6 plays a part in the immune, endocrine, nervous, and hematopoietic systems, in addition to bone metabolism, regulation of blood pressure and inflammation. Osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. Furthermore, IL-6 is an important mediator of fever and of the acute phase response which is the body's rapid attempt to restore homeostasis after tissue injury, infection, neoplastic growth, or immunological disturbance. In addition, IL-6 can be released into circulation in response to various stimuli including PAMPs (pathogen-associated molecular patterns) and cortisol, a hormone produced by the human body under psychologically stressful conditions. In its role as an anti-inflammatory myokine, IL-6 precedes the appearance of other cytokines in the circulation, is notably elevated with exercise, and is mediated by both its inhibitory effects on TNF-α and IL-1, and activation of IL-1ra and IL-10. IL-6 signals through a cell-surface type I cytokine receptor complex formed by the binding of IL-6 to IL-6R, forming a binary complex, which in turn combines with GP130 to transduce extracellular signaling by the activation STAT3. Hence, it is thought that blocking the interaction between IL-6 and GP130 may have therapeutic potential via the inhibition of the IL-6/GP130/STAT3 signaling pathway. Moreover, IL-6 initiates the inflammatory and auto-immune processes in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's disease, rheumatoid arthritis, cancer, and various others. Thus, there is an interest in the therapeutic potential of anti-IL-6 mAbs.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse IL-6 was determined in a cell proliferation assay. The expected ED50=0.02-0.06 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
mfptsqvrrg dftedttpnr pvyttsqvgg lithvlweiv emrkelcngn sdcmnnddal aennlklpei qrndgcyqtg ynqeicllki ssglleyhsy leymknnlkd nkkdkarvlq rdtetlihif nqevkdlhki vlptpisnal ltdklesqke wlrtktiqfi lksleeflkv tlrstrqt
N-terminal Sequence Analysis
Phe25
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IL-6 is Mr 21.8 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and is supplied in a solution of 50 mM sodium acetate (NaOAc) and EDTA.
Storage and Stability
This protein is stable six (6) to twelve (12) months when stored at 2°C to 8°C. Do not Freeze This Product.
Country of Origin
USA
Shipping
Next Day Ice Packs
PubMed
NCBI Gene Bank

References & Citations

1. Febbraio, MA. and Pedersen, BK. (2005) Exerc Sport Sci Rev. 33(3):114-9.
2. Baier, M. et al. (1997) Proc Natl Acad Sci U S A. 94(10):5273-7.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.