Anti-Rat IL-6 (Clone 53325)

Anti-Rat IL-6 (Clone 53325)

Product No.: I-674

[product_table name="All Top" skus="I-674"]

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Clone
53325
Target
IL-6
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
BSF2, HPGF, HSF, IFNB2, MGI-2, HGF, B Cell Differentiation Factor [BCDF], Interleukin-6
Isotype
IgG2b
Applications
ELISA Cap

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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Mouse
Immunogen
Purified Recombinant Rat IL-6 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-257 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-213.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 53325 recognizes an epitope on rat IL-6.
Background
IL-6 is a pleotropic 26 kD protein that can act as both a pro-inflammatory cytokine and an anti-inflammatory myokine, a form of cytokine produced in muscle cells that participates in tissue regeneration and repair, maintenance of healthy bodily functioning, and homeostasis within the immune system. IL-6 plays a part in the immune, endocrine, nervous, and hematopoietic systems, in addition to bone metabolism, regulation of blood pressure and inflammation. Osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. Furthermore, IL-6 is an important mediator of fever and of the acute phase response which is the body's rapid attempt to restore homeostasis after tissue injury, infection, neoplastic growth, or immunological disturbance. In addition, IL-6 can be released into circulation in response to various stimuli including PAMPs (pathogen-associated molecular patterns) and cortisol, a hormone produced by the human body under psychologically stressful conditions. In its role as an anti-inflammatory myokine, IL-6 precedes the appearance of other cytokines in the circulation, is notably elevated with exercise, and is mediated by both its inhibitory effects on TNF-α and IL-1, and activation of IL-1ra and IL-10. IL-6 signals through a cell-surface type I cytokine receptor complex formed by the binding of IL-6 to IL-6R, forming a binary complex, which in turn combines with GP130 to transduce extracellular signaling by the activation STAT3. Hence, it is thought that blocking the interaction between IL-6 and GP130 may have therapeutic potential via the inhibition of the IL-6/GP130/STAT3 signaling pathway. Moreover, IL-6 initiates the inflammatory and auto-immune processes in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's disease, rheumatoid arthritis, cancer, and various others. Thus, there is an interest in the therapeutic potential of anti-IL-6 mAbs.

Antigen Details

PubMed
Research Area
Other Molecules

References & Citations

1. Pederson, BK. et al. (2005) Exerc Sport Sci Rev 33: 114
2. Baier, M. et al. (1997) Proceedings of the National Academy of Sciences (USA) 94: 5273
Elisa Sandwich Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
I-674
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.