Anti-Human IL-6 (Clone 6708)

Pricing & Details

Product No.I-633
Clone
6708
Protein
IL-6
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Interleukin-6, BSF2, HPGF, HSF, IFNB2, MGI-2, HGF, B Cell Differentiation Factor [BCDF]
Isotype
Mouse
IgG1
Applications
ELISA Cap
,
IHC
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-633-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
I-633-100 µg
100 µg
$399.00
In stock
Max:
Min: 1
Step: 1
I-633-500 µg
500 µg
$849.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human IL-6 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-255 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-188.
Western Blotting: To detect Human IL-6 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-6 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-188.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human IL-6 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-6 (at 2.5 ng/ml) is 0.05 - 0.15 µg/ml.
Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 6708 recognizes an epitope on human IL-6. In Western blots, this antibody shows no cross-reactivity with rmIL-6, rhIL-6 R, rhOSM, rhLIF, rhIL-11, rhgp130, or rhCNTF.
Background
IL-6 is a pleotropic 26 kD protein that can act as both a pro-inflammatory cytokine and an anti-inflammatory myokine, a form of cytokine produced in muscle cells that participates in tissue regeneration and repair, maintenance of healthy bodily functioning, and homeostasis within the immune system. IL-6 plays a part in the immune, endocrine, nervous, and hematopoietic systems, in addition to bone metabolism, regulation of blood pressure and inflammation. Osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. Furthermore, IL-6 is an important mediator of fever and of the acute phase response which is the body's rapid attempt to restore homeostasis after tissue injury, infection, neoplastic growth, or immunological disturbance. In addition, IL-6 can be released into circulation in response to various stimuli including PAMPs (pathogen-associated molecular patterns) and cortisol, a hormone produced by the human body under psychologically stressful conditions. In its role as an anti-inflammatory myokine, IL-6 precedes the appearance of other cytokines in the circulation, is notably elevated with exercise, and is mediated by both its inhibitory effects on TNF-α and IL-1, and activation of IL-1ra and IL-10. IL-6 signals through a cell-surface type I cytokine receptor complex formed by the binding of IL-6 to IL-6R, forming a binary complex, which in turn combines with GP130 to transduce extracellular signaling by the activation STAT3. Hence, it is thought that blocking the interaction between IL-6 and GP130 may have therapeutic potential via the inhibition of the IL-6/GP130/STAT3 signaling pathway. Moreover, IL-6 initiates the inflammatory and auto-immune processes in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's disease, rheumatoid arthritis, cancer, and various others. Thus, there is an interest in the therapeutic potential of anti-IL-6 mAbs.

Antigen Details

Protein
PubMed

References & Citations

1. Pederson, BK. et al. (2005) Exerc Sport Sci Rev 33: 114
2. Baier, M. et al. (1997) Proceedings of the National Academy of Sciences (USA) 94: 5273
Elisa Sandwich Protocol
IHC
General Western Blot Protocol

Formats Available

Prod No.
Description
I-633
Products are for research use only. Not for use in diagnostic or therapeutic procedures.