Recombinant Mouse LIGHT

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Pricing & Details

Product. No.L102
Alternate Names
TNFSF14, HVEM-L, TR2, CD258, LTg
Expression Host
NS0 cells
Species
Mouse
Prod No.
Size
Price
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Qty
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L102-25 µg
25 µg
$359.00
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Min: 1
Step: 1

Background

LIGHT is a type II transmemebrane protein and a member of the TNF cytokine family also referred to as TNFSF14 and it is identical with HVEM ligand. LIGHT is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells. LIGHT binds to three receptors; the herpes virus entry mediator type A receptor (HEVM), LTbR and a decoy receptor (DcR3).1

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse LIGHT was determined by a cytotoxicity assay using HT29 cells, a human colon adenocarcinoma cell line (Zhai, Y. et al., 1998, J. Clin. Invest. 102:1142). The expected ED50 for this effect is typically 2 - 5 μg/ml in the presence of 10 μg/ml of a cross-linking antibody PN:246 and 1 ng/ml of rhIFN-γ.
Fusion Protein Tag
Histidine Tag
Expression Host
NS0 cells
Protein Accession No.
Q9QYH9
Amino Acid Sequence
hhhhhhhhhh gggsgggsgg gsiegrdggk gswekliqdq rshqanpaah ltganaslig iggpllwetr lglaflrglt yhdgalvtme pgyyyvyskv qlsgvgcpqg langlpithg lykrtsrypk elellvsrrs pcgranssrv wwdssflggv vhleageevv vrvpgnrlvr prdgtrsyfg afmv
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse LIGHT is Mr 21 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 24-34 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with BSA as a carrier protein pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Guo, Y. et al. (2005) Hybridoma (Larchmt). 24(6):309-13.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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