Purified Recombinant Human LIGHT (Accession # O43557)
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
This lyophilized, biotin polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve (12) months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human LIGHT this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human LIGHT is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L202.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 50 ng/ml when used in conjunction with PN:L219 as the capture antibody at 4 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human LIGHT.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Human LIGHT recognizes Human LIGHT. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
LIGHT is a type II transmemebrane protein and a member of the TNF cytokine family also referred to as TNFSF14 and it is identical with HVEM ligand. LIGHT is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells. LIGHT binds to three receptors; the herpes virus entry mediator type A receptor (HEVM), LTbR and a decoy receptor (DcR3).1
NCBI Gene Bank ID
References & Citations
1. Guo, Y. et al. (2005) Hybridoma (Larchmt). 24(6):309-13.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.