Anti-Human LIGHT

Pricing & Details

Product No.L158
Protein
LIGHT
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
TNFSF14, HVEM-L, TR2, CD258, LTg
Applications
ELISA Indirect
,
IHC
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
L158-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
L158-100 µg
100 µg
$445.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
NS0-Derived Recombinant Human LIGHT Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:G505. The detection sensitivity of this indirect ELISA for Human LIGHT is approximately 0.2 ng/well.
Western Blotting: To detect Human LIGHT this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human LIGHT is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human LIGHT bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human LIGHT (at 1 ng/ml) is 5 - 20 µg/ml.
Immunohistochemistry: Suitable for use at concentration of 3-15 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human LIGHT recognizes Human LIGHT. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
LIGHT is a type II transmemebrane protein and a member of the TNF cytokine family also referred to as TNFSF14 and it is identical with HVEM ligand. LIGHT is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells. LIGHT binds to three receptors; the herpes virus entry mediator type A receptor (HEVM), LTbR and a decoy receptor (DcR3).1

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Guo, Y. et al. (2005) Hybridoma (Larchmt). 24(6):309-13.
Indirect Elisa Protocol
IHC
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.