Recombinant Mouse MCP-5

Monocyte chemotactic protein 5 (MCP-5), also known as CCL 12, is a novel and potent monocyte active chemokine that is involved in allergic inflammation and the host response to pathogens.¹ It is also a potent chemoattractant for peripheral blood monocytes and induces a calcium flux in peripheral blood mononuclear cells.²

The biological activity of Mouse MCP-5 was determined by its ability to chemoattract 2 day cultured human monocytes (Matsushima, K. et al., 1989, J. Exp. Med. 169:1485) and by its ability to chemoattract hCCR2A transfected mouse BaF/3 cells. The expected ED₅₀ for these effects are typically 0.02 - 0.06 μg/ml and 0.015 - 0.075 μg/ml, respectively.

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Gly23

Lyophilized

The predicted molecular weight of Recombinant Mouse MCP-5 is Mr 9.3 kDa.

9.3

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Luster, AD. et al. (1997) J Exp Med. 185: 99
2. Jia, GQ. et al. (1996) J Exp Med. 184: 1939