Anti-Mouse MCP-5 (Clone 63205)

Pricing & Details

Product No.C1413
Clone
63205
Protein
MCP-5
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
SCYA12, CCL12, Monocyte Chemoattractant Protein-5
Isotype
Rat
IgG2a
Applications
ELISA Cap
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1413-25 µg
25 µg
$195.00
In stock
Max:
Min: 1
Step: 1
C1413-100 µg
100 µg
$425.00
In stock
Max:
Min: 1
Step: 1
C1413-500 µg
500 µg
$925.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse MCP-5 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:M128 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:M163.
Western Blotting: To detect Mouse MCP-5 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MCP-5 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M163.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse CCL12 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CCL12 (at 0.2 ng/ml) is 5-15 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse CCL12 (Clone 63205) recognizes an epitope on Mouse CCL12. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Monocyte chemotactic protein 5 (MCP-5), also known as CCL 12, is a novel and potent monocyte active chemokine that is involved in allergic inflammation and the host response to pathogens.1 It is also a potent chemoattractant for peripheral blood monocytes and induces a calcium flux in peripheral blood mononuclear cells.2

Antigen Details

Protein
PubMed

References & Citations

1. Luster, AD. et al. (1997) J Exp Med. 185: 99
2. Jia, GQ. et al. (1996) J Exp Med. 184: 1939
Elisa Sandwich Protocol
General Western Blot Protocol

Formats Available

Prod No.
Description
C1413
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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