Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse MCP-5 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828472 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:M128 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:M163. Western Blotting: To detect Mouse MCP-5 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MCP-5 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M163. Other Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse CCL12 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CCL12 (at 0.2 ng/ml) is 5-15 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse CCL12 (Clone 63205) recognizes an epitope on Mouse CCL12. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Monocyte chemotactic protein 5 (MCP-5), also known as CCL 12, is a novel and potent monocyte active chemokine that is involved in allergic inflammation and the host response to pathogens.1 It is also a potent chemoattractant for peripheral blood monocytes and induces a calcium flux in peripheral blood mononuclear cells.2 Antigen DetailsProtein PubMed References & Citations1. Luster, AD. et al. (1997) J Exp Med. 185: 99 2. Jia, GQ. et al. (1996) J Exp Med. 184: 1939 Technical Protocols  |
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