Recombinant Mouse RANK Ligand

RANKL (Receptor Activator for Nuclear Factor κ B Ligand), also known as TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and ODF (osteoclast differentiation factor), is a member of the tumor necrosis factor family cytokine.¹ RANKL is essential for osteoclast formation, activation, and survival.² RANKL through interaction with its receptor RANK can modulate matrix degradation and inflammation.³ RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy. Consequently, novel drugs specifically targeting RANK, RANKL, and their signaling pathways in osteoclasts are expected to revolutionize the treatment of various ailments associated with bone loss, such as arthritis, periodontal disease, cancer metastases, and osteoporosis.⁴

The biological activity of Mouse RANKL was determined by its ability to induce osteoclast differentiation on mouse splenocytes. The expected ED₅₀ for this effect is typically 5-15 ng/ml in the presence of 2.5 μg/ml of a cross-linking antibody PN:P246.

Histidine Tag

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His

Solution

The predicted molecular weight of Recombinant Mouse RANKL is Mr 28 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 36 kDa.

28

This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

This recombinant protein solution is stable for up to twelve months when stored at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Upon thawing, this protein, in the presence of a carrier protein, it can be stored at 2° - 8° C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20° C to -70° C in a manual defrost freezer. Avoid repeated freeze-thaw cycles..

Dry Ice

1. Takayanagi, H. et al. (2003) Arthritis Res Ther. 5: 20
2. Kostenuik, PJ. et al. (2005) Endocrinology 146: 3235
3. Holven, KB. et al. (2009) Stroke 40: 241
4. Penninger, JM. et al. (2008) Ann N Y Acad Sci. 1143: 123