UltraBlock-BSA™ Blocking Buffer ‘Ready to Use’

UltraBlock-BSA™ Blocking Buffer ‘Ready to Use’

Product No.: B395

[product_table name="All Top" skus="B395"]

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Product Type
Substrate and Assay Reagents
Applications
ELISA
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WB

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Select Product Size
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Product Details

Storage and Handling
Store at 2-8° C. Do Not Freeze
Country of Origin
USA
Shipping
Next Day 2-8°C
Expiration Date
Stable for a minimum of 2 years after receipt when properly stored at 2-8°C

Description

Background
Protein containing solutions are commonly used in ELISA and other immunoassays to block excess binding sites and reduce non-specific binding of secondary reagents. Bovine Serum Albumin (BSA) has long been the choice for many scientists attempting to reduce non-specific binding in immunoassays. UltraBlock-BSATM utilizes special chemistry to enhance the solubility and stabilize the BSA. UltraBlock-BSATM readily blocks and excess reagent easily washes away providing ideal blocking without lingering residual protein that can interfere with optimal signal. An additional benefit of UltraBlock-BSATM is that it provides a long term stabilizing environment for the antigen, or antibody coated on the ELISA plate. long been the choice for many scientists attempting to reduce non-specific binding in immunoassays. UltraBlock-BSATM utilizes special chemistry to enhance the solubility and stabilize the BSA. UltraBlockBSATM readily blocks and excess reagent easily washes away providing ideal blocking without lingering residual protein that can interfere with optimal signal. An additional benefit of UltraBlock-BSATM is that it provides a long term stabilizing environment for the antigen, or antibody coated on the ELISA plate.
Benefits
• Ease of Use – A one component “Ready Use” formulation increases assay efficiency.
• Extended Shelf Life – UltraBlock-BSATM is stable for a minimum of 2 years when stored at 2° - 8°C.
Directions for Use
UltraBlock-BSATM is a ready to use solution that needs no preparation or dilution. Once you have coated your micro ELISA plate(s) with capture antibody or antigen, aspirate or dump out the coating solution and wash the plate 2-4 times. Pipette a volume greater than the coating solution volume, but not to exceed 350 µl of UltraBlock-BSATM per well and let incubate for 2 – 3 hours in an incubator at 37°C.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Related Protocols

Elisa Sandwich Protocol
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.