Anti-Mouse Vγ2 TCR – Purified in vivo GOLD™ Functional Grade

Anti-Mouse Vγ2 TCR – Purified in vivo GOLD™ Functional Grade

Product No.: T353

[product_table name="All Top" skus="T353"]

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Clone
UC3-10A6
Target
Vγ 2 T-Cell Receptor
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Vγ2 T-cell receptor, T cell receptor gamma 2
Isotype
IgG
Applications
Depletion
,
FC
,
in vivo
,
IP

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Armenian Hamster
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 106 cells in a 100 µl total staining volume, followed by Goat-anti-Hamster IgG (PN:A237). Each lot of this antibody is quality control tested.
Additional Applications Reported In Literature ?
Depletion:The UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This antibody reacts with Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes.
Antigen Distribution
Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung.
Background
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.

Antigen Details

Ligand/Receptor
Antigens
Function
Studies suggest that these cells recognize bacterial ligands and some tumor cells.
Research Area
Adaptive Immunity
.
Immunology

References & Citations

1. Hedrick, SM. et al. (1990) Nature. 343:714
Depletion
Flow Cytometry
in vivo Protocol
Immunoprecipitation Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.