Anti-Mouse Vγ2 TCR – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse Vγ2 TCR – Purified in vivo PLATINUM™ Functional Grade

Product No.: T704

[product_table name="All Top" skus="T704"]

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Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Vγ2 T-cell receptor, T cell receptor gamma 2
in vivo

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Product Size
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Antibody Details

Product Details

Reactive Species
Host Species
Armenian Hamster
G8 mouse T cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
≥98% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry:It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 106 cells in a 100 µl total staining volume, followed by Goat PN:A237.
Additional Applications Reported In Literature ?
Depletion: The UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Armenian Hamster Anti-Mouse Vγ2 TCR recognizes an epitope on Mouse Vγ2 TCR. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern.
Antigen Distribution
Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung.
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules. Clone UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo.

Antigen Details

NCBI Gene Bank ID
Research Area
Adaptive Immunity

References & Citations

1. Hedrick, SM. et al. (1990) Nature. 343:714
Flow Cytometry
in vivo Protocol
Immunoprecipitation Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.