G8 mouse T cells
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
The antibody was purified by affinity chromatography and conjugated to PE under optimal conditions.
Storage and Handling
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day Ambient
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. Each lot of this antibody is quality control tested.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
This antibody reacts with Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes.
Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung.
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.
Reported to play a role in a previously unrecognized form of immunological surveillance for stress-induced self-antigens.
References & Citations
Hedrick, S. M. et al. (1990) Nature. 343:714
Products are for research use only. Not for use in diagnostic or therapeutic procedures.