Anti-Mouse Vγ2 TCR – Purified in vivo GOLD™ Functional Grade
Anti-Mouse Vγ2 TCR – Purified in vivo GOLD™ Functional Grade
Product No.: T353
Clone UC3-10A6 Protein Vγ 2 T-Cell Receptor Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Vγ2 T-cell receptor, T cell receptor gamma 2 Isotype Armenian Hamster IgG Applications Depletion , FC , in vivo , IP |
Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Armenian Hamster Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2894354 Applications and Recommended Usage? Quality Tested by Leinco FC: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 106 cells in a 100 µl total staining volume, followed by Goat-anti-Hamster IgG (PN:A237). Each lot of this antibody is quality control tested. Other Applications Reported In Literature ? Depletion:The UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody reacts with Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes. Antigen Distribution Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung. Background The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules. Antigen DetailsProtein Ligand/Receptor Antigens Function Studies suggest that these cells recognize bacterial ligands and some tumor cells. PubMed Research Area Adaptive Immunity . Immunology References & Citations1. Hedrick, SM. et al. (1990) Nature. 343:714 Technical Protocols   |
Formats Available
Prod No. | Description |
|---|---|
T554 |
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T212 |
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T219 |
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T353 |
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T211 |
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T213 |
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T594 |
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T704 |
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T210 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.