Anti-Mouse CD11a (Clone I21/7) – Purified in vivo GOLD™ Functional Grade

Clone I21/7 is an anti-mouse CD11a monoclonal antibody specifically designed for in vivo mouse studies, where it serves as a powerful tool for investigating immune cell function and interactions.

Target and Specificity

Clone I21/7 specifically recognizes mouse CD11a (also known as LFA-1?), which is present on thymocytes, blood lymphocytes, bone marrow cells, and other immune cell populations. CD11a is a critical component of the LFA-1 integrin complex that plays essential roles in immune cell adhesion, migration, and activation.

Primary Applications in Research

Immune Cell Depletion and Functional StudiesThe antibody is commonly used to deplete or block CD11a-expressing cells in vivo, allowing researchers to study the functional importance of these cells in various biological processes. This is particularly valuable for understanding immune responses, inflammation, and cell trafficking.

Transplantation ResearchClone I21/7 has proven especially useful in stem cell and transplantation studies. In mouse clone models designed to evaluate immunogenicity and therapeutic potential of induced pluripotent stem cells (iPSCs), this antibody helps researchers investigate immune rejection mechanisms. These studies are crucial for understanding how to achieve truly autologous transplantation and minimize immune responses.

Cancer Immunotherapy StudiesThe antibody is employed in cancer research to understand immune cell infiltration and function within tumors. Studies examining combination therapies with checkpoint inhibitors like PD-1 and CTLA-4 antibodies utilize CD11a targeting to analyze how different immune cell populations contribute to antitumor responses.

Technical Advantages for In Vivo Use

Low Endotoxin FormulationModern preparations of clone I21/7 are manufactured as low endotoxin antibodies (less than 1.0 EU/mg) in cGMP-compliant facilities, which is essential for in vivo applications to avoid inflammatory responses that could confound experimental results. Ultra-low endotoxin versions (less than 0.5 EU/mg) are also available for even more sensitive applications.

High Purity StandardsThe antibody is produced at high purity levels (98% or higher) using ISO Quality Standard 9001:2015 manufacturing processes, ensuring consistent and reliable results in experimental studies.

Clone I21/7 represents an essential tool for immunologists and stem cell researchers conducting in vivo mouse studies, particularly when precise manipulation of CD11a-expressing immune cell populations is required to understand complex biological processes or evaluate therapeutic interventions.

The correct storage temperature for a sterile packaged clone such as I21/7 depends on its intended use and packaging, but for sterile plant clones (e.g., in tissue culture or similar setups), refrigerator storage at +2°C to +8°C (36°F to 46°F) is generally recommended for maintaining viability over extended periods.

For general sterile packaged medical or laboratory items, storage should be in a dry, clean, and protected environment at a maximum room temperature of 25°C (77°F), away from light, dust, and any unsterile objects. However, plant tissue cultures and many vegetative clones are specifically best preserved at refrigerator temperatures rather than just room temperature for optimal longevity.

• Plant clone storage: +2°C to +8°C (in a refrigerator)
• General sterile medical item storage: Up to 25°C, dry, clean, away from light

If your "clone I21/7" is a plant tissue culture or vegetative cutting, use refrigerator storage (2-8°C). If it is a sterile packaged medical device, use controlled room temperature (up to 25°C), dry, and clean conditions.

If you can clarify the type of clone or provide the manufacturer's guidelines, more specific instructions may be possible.

Other commonly used antibodies or cytokines combined with IL-21 or IL-7 (or I21/7, assuming this is shorthand for IL-21/IL-7) in the literature include IL-15, IL-2, and various immune checkpoint inhibitors (such as anti–PD-1 antibodies).

Key details and context:

• IL-15 is frequently co-formulated with IL-7 or IL-21 in antibody-cytokine fusion proteins for immunotherapy. These trifunctional constructs (e.g., IL-15+IL-7 or IL-15+IL-21) aim to enhance T cell proliferation and cytotoxicity beyond single-cytokine approaches.
• IL-2 is another cytokine often engineered with antibodies (or in fusion constructs) for biased immune cell activation. Pairing cytokines like IL-2 with biasing antibodies improves efficacy and safety, and similar logic applies to other common gamma chain cytokines such as IL-7 and IL-21.
• Immune checkpoint inhibitors (notably anti–PD-1 antibodies) are increasingly paired or fused with cytokines such as IL-21 to boost antitumor activity in next-generation therapies.

Other fusion and combination strategies include:

• Monoclonal antibodies targeting cancer antigens can be fused with cytokines like IL-21, IL-7, or IL-15 to localize immune stimulation to the tumor site.
• Antibody–drug conjugates and bispecific antibodies may also be combined or engineered to include cytokines for synergistic effects.

In summary, IL-15, IL-2, and anti–PD-1 antibodies are among the most commonly used proteins with IL-21 or IL-7 in combination immunotherapy research.

There are no clear or direct key findings from scientific literature that specifically reference a "clone I21/7" based on the search results provided. None of the cited sources identify or discuss "clone I21/7" as an experimental model, cell line, antibody, genetic variant, or scientific protocol.

Context and supporting details:

• The search results address broader topics such as cloning definitions, major studies on reproductive and embryonic stem cell cloning, citation practices, and the history of cloning methodologies.
• If "clone I21/7" is a specific antibody, cell line, or genetic clone, these results do not mention it. Therefore, there are no summarizable key findings from published scientific citations specifically related to "clone I21/7".
• If you are seeking information about a specific clone (for example, in immunology or molecular biology), additional context or alternate terminology might help locate the relevant literature.

Inference: The absence of mention suggests either "clone I21/7" is a highly specialized or new entity, or it may be referenced differently in the literature. For accurate results, providing the organism, field, or product type (e.g., "antibody I21/7," "cell line I21/7," or related application) would facilitate targeted findings.