Product Datasheet
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Anti-Mouse CD3ε (CD3ε) Purified Low Endotoxin Functional Formulation

(CD3, T3)
Monoclonal Antibody


Prod. No.:C1758
Clone:145-2C11
Isotype:Armenian Hamster IgG
Conc.:1.0 mg/ml
Pkg. Size:0.5 mg, 1.0 mg, 5.0 mg, 25 mg, 100 mg
Storage:Sterile 2-8°C Detailed storage instructions below.
Description
Specificity:
Armenian Hamster Anti-Mouse CD3ε (CD3ε) (Clone 145-2C11) recognizes an epitope on Mouse CD3ε. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.

Background:
CD3ε is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Known Species Cross Reactivity: Ms+
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purity
>98% by SDS-PAGE and HPLC
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
This Low Endotoxin Functional Formulation conjugate is stable when stored at 2-8°C. Do not freeze. This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), FC, IP, WB, In vivo Depletion, B
Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Anti-Mouse CD3ε-Biotin (Leinco Prod. No.: C208)
PN:
Anti-Mouse CD3ε Purified (Leinco Prod. No.: C207)
Anti-Mouse CD3ε Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C219)
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