Anti-Mouse CD3ε – Purified In vivo PLATINUM™ Functional Grade

Pricing & Details

Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD3, T3
Armenian Hamster
Prod No.
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C2280-1.0 mg
1.0 mg
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C2280-5.0 mg
5.0 mg
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C2280-25 mg
25 mg
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C2280-50 mg
50 mg
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C2280-100 mg
100 mg
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Antibody Details

Reactivity Species
Host Species
Armenian Hamster
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Product Concentration
1.0 - 5.0 mg/ml
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells
Other Applications Reported In Literature ?
IHC (Frozen) The suggested concentration for this 145-2C11 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.

Additional Reported Applications For Relevant Conjugates
IF Staining
IHC (Paraffin)
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications.


Clone 145-2C11 recognizes an epitope on mouse CD3ε.
Antigen Distribution
CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein Family
Peptide antigen/MHC-complex
NCBI Gene Bank ID
Flow Cytometry
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.