Anti-Mouse CD3ε – Biotin

Pricing & Details

  
Clone
145-2C11
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD3, T3
Isotype
Armenian Hamster
IgG
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2235-100 ug
100 ug
$40.00
In stock
C2235-200 µg
200 µg
$110.00
In stock
  

Antibody Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
Purified Recombinant Mouse CD3ε (>98%)
Product Concentration
0.5 mg/ml
Formulation
This Biotin conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3ε. A suggested method would be to stain cells expressing CD3ε with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:A104.
Additional Reported Applications For Relevant Conjugates
IHC (Frozen)
IP
Act
B
WB
ICC
Depletion
IF Staining
IHC (Paraffin)
For specific conjugates of this clone, review literature for suggested application details.

Description

Specificity
Clone 145-2C11 recognizes an epitope on mouse CD3ε.
Antigen Distribution
CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein Family
CD3ε
Ligand/Receptor
Peptide antigen/MHC-complex
NCBI Gene Bank ID
Flow Cytometry
  

Formats Available

  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.