Anti-Mouse CD3ε – APC

Pricing & Details

  
Clone
145-2C11
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD3, T3
Isotype
Armenian Hamster
IgG
Applications
FA
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2036-50 ug
50 ug
$60.00
In stock
C2036-100 µg
100 µg
$98.00
In stock
  

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Product Concentration
0.1 mg/ml
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Allophycocyanin (APC) conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Applications and Recommended Usage
Flow Cytometry: This antibody can be used at a concentration of ≤ 0.5 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
IHC (Frozen)
IP
Act
B
WB
ICC
Depletion
IF Staining
IHC (Paraffin)
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Excitation Laser
Red Laser (633 nm)

Description

Specificity
Clone 145-2C11 recognizes an epitope on mouse CD3ε.
Antigen Distribution
CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein
CD3ε
Ligand/Receptor
Peptide antigen/MHC-complex
PubMed
145-2C11+CD3ε
NCBI Gene Bank ID

References & Citations

Flow Cytometry
  

Formats Available

  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.