Anti-Mouse CD3ε – Purified

Pricing & Details

  
Clone
145-2C11
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD3, T3
Isotype
Armenian Hamster
IgG
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1757-500 µg
500 µg
$65.00
In stock
  

Antibody Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Product Concentration
0.5 mg/ml
Formulation
This Purified conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
IHC (Frozen) The suggested concentration for this 145-2C11 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
IP
Act
B
WB
ICC

Additional Reported Applications For Relevant Conjugates
Depletion
IF Staining
IHC (Paraffin)
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications.

Description

Specificity
Clone 145-2C11 recognizes an epitope on mouse CD3ε.
Antigen Distribution
CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein Family
CD3ε
Ligand/Receptor
Peptide antigen/MHC-complex
NCBI Gene Bank ID
Flow Cytometry
Immunohistochemistry
Immunoprecipitation Protocol
General Western Blot Protocol
  

Formats Available

  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.