Antibody DetailsReactivity Species Mouse Host Species Armenian Hamster Immunogen H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37 Product Concentration 0.5 mg/ml Formulation This Purified conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Applications and Recommended Usage ? (Quality Tested by Leinco) FC The suggested concentration for this 145-2C11 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Other Applications Reported In Literature ? IHC (Frozen) The suggested concentration for this 145-2C11 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application. IP Act B WB ICC Additional Reported Applications For Relevant Conjugates Depletion IF Staining IHC (Paraffin) For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. DescriptionSpecificity Clone 145-2C11 recognizes an epitope on mouse CD3ε. Antigen Distribution CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen DetailsProtein Family CD3ε Ligand/Receptor Peptide antigen/MHC-complex NCBI Gene Bank ID UniProt.org Technical Protocols |
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