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Anti-Mouse CD4 (CD4) DyLight® 488

(CD4mut, L3T4, Ly-4, Cd4, CD4 Antigen)
Monoclonal Antibody


Prod. No.:C1636
Clone:GK1.5
Isotype:Rat IgG2b
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 100 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.

Background:
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.1

Reporter Molecule:
DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Host Species
Rat
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 0.06 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Hendrickson WA et al. (1994) Structure 2: 59
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