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Anti-Mouse CD4 (CD4) Biotin

(CD4mut, L3T4, Ly-4, Cd4, CD4 Antigen)
Monoclonal Antibody


Prod. No.:C212
Clone:GK1.5
Isotype:Rat IgG2b
Conc.:0.5 mg/ml
Pkg. Size:100 µg, 500 µg
Starting at:$50.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.

Background:
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.1

Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
IHC (Frozen), FC
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD4. A suggested method would be to stain cells expressing CD4 with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by UltraAvidin™- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Hendrickson WA et al. (1994) Structure 2: 59
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