Anti-Transcription Factor IIB (TFIIB) Antibody (Clone IIB8)

Partially purified recombinant human transcription factor IIB (TFIIB, Promega Corp.).

PBS, pH 7.4.

Liquid

Purified by Protein G affinity chromatography

These antibodies are stable for at least one (1) year at -20°C to -70 C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Next Day 2-8°C

Mouse Monoclonal Antibody specific to an epitope within amino acids 52-105 of human and mouse Transcription Factor IIB (TFIIB).

TFIIB (also known as GTF2B, TF2B, or general transcription factor IIB) is a ubiquitously expressed transcription factor that plays a pivotal role in the formation of the transcription pre-initiation complex. It facilitates the recruitment of RNA polymerase II by acting as a molecular bridge between TFIID, which is bound at the promoter, and the polymerase itself.

In addition to its interactions with TFIID and TFIIA, TFIIB engages directly with the core promoter region through two distinct DNA-binding domains, supporting accurate positioning and stabilization of the transcription machinery. TFIIB is also thought to be a regulatory target for transcriptional activators that enhance pre-initiation complex assembly.

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-rPubMed:10318856, PubMed:10619841, PubMed:12931194, PubMed:1517211, PubMed:16230532, PubMed:1876184, PubMed:1946368, PubMed:24441171, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:7675079, PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329}.

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