Anti-Transcription Factor IIB Antibody (70103)
Anti-Transcription Factor IIB Antibody (70103)
Product No.: 70103
- -
- -
Clone IIB8 Target Transcription Factor IIB Formats AvailableView All Product Type Monoclonal Alternate Names EC 2.3.1.48, General transcription factor TFIIB, S300-II Isotype Mouse IgG2a Applications ELISA , WB , Gel Shift , Inhibition |
- -
- -
Antibody DetailsProduct DetailsReactive Species Human/Mouse Host Species Mouse Immunogen Partially purified recombinant human transcription factor IIB (TFIIB, Promega Corp.). Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling These antibodies are stable for at least one (1) year at -20°C to -70 C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco This antibody may be used in ELISA, Western blot, or electrophoretic mobility (gel shift) assays to identify human TFIIB. It can also immuno-deplet TFIIB from cell nuclear extracts and inhibit transcription of TFIIB from cell nuclear extracts. This antibody is polyol-responsive and can be used for gentle purification of TFIIB. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Monoclonal Antibody specific to an epitope within amino acids 52-105 of human
and mouse Transcription Factor IIB (TFIIB). Function General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-rPubMed:10318856, PubMed:10619841, PubMed:12931194, PubMed:1517211, PubMed:16230532, PubMed:1876184, PubMed:1946368, PubMed:24441171, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:7675079, PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329}. NCBI Gene Bank ID UniProt.org Research Area Protein Transcription References & CitationsThompson NE and Burgess RR. 1994. Protein Expression Purification 5: 468- 475 Thompson NE et al. 1995. J Biol Chem 270: 4735-4740. Technical Protocols  Gel Shift Inhibition Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
