Anti-8-oxo-Adenosine (8-oxoA) DNA/RNA Damage Monoclonal Antibody
(Clone 6E4) (12503)

Anti-8-oxo-Adenosine (8-oxoA) DNA/RNA Damage Monoclonal Antibody
(Clone 6E4) (12503)

Product No.: 12503

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Clone
6E4
Target
8-oxoA
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
-8-oxoAdenine, -8-oxo-Adenosine, -8-oxoA, -8-hydroxyadenosine
Isotype
Mouse IgG1κ
Applications
ELISA

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Select Product Size

Data

Graph showing the effectiveness of Anti-8-oxo-Adenosine DNA/RNA Damage Monoclonal Antibody (12503) in detecting 8-oxoA in DNA. Two plots compare ssDNA/dsDNA and DNA base mispairs, with curves indicating different concentrations and competitor levels.Selectivity and Specificity of 6E4 in RNA:
* Binds 8-oxoA in single and double stranded RNA
* Recognizes 8-oxoA in RNA base mispairs with standard geometry
Graph showing 8-oxoA recognition in RNA. Two sections compare ssRNA and dsRNA, and RNA base mispairs. Curves depict binding efficiency of Anti-8-oxo-Adenosine DNA/RNA Damage Monoclonal Antibody (12503) with varying competitors.Selectivity and Specificity of 6E4 in DNA:
* Binds 8-oxoA in single and double stranded DNA
* Recognizes 8-oxoA in all DNA base mispairs
Clone 6E4 8-oxoA Specificity
Determined by a competitive inhibition ELISA with nucleoside variants. The % B/Bo is plotted as a function of the concentration (nM) of the indicated competitor. 100% B/Bo indicates no inhibition by the indicated competitor.
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
8-OxoA conjugated to KLH
Product Concentration
Lot Specific
Formulation
PBS, pH 7.4
State of Matter
Liquid
Product Preparation
Protein G Affinity Chromatography
Storage and Handling
Store antibody at -20°C to -80°C. Avoid multiple freeze-thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Anti-8-oxoA is a mouse monoclonal antibody targeting oxidative damage in RNA, DNA, ATP and other nucleotide precursors and degradation products.
Background
This target is a byproduct of oxidative damage to the adenosine nucleobase in DNA, RNA, and nucleotides such as ATP. In DNA, 8-oxoA mispairs may reduce fidelity of replication and cause transcriptional errors by RNA pol II. In RNA, 8-oxoA damage leads to attenuation of mRNA translation. Specialized base excision repair machinery and nucleotide sanitation processes are present to reduce the mutagenic consequences of 8-oxoA.

This antibody is the intellectual property of CymbaX, sequencing of this antibody is prohibited.
Research Area
DNA/RNA Damage

References & Citations

Daniel J. Tew, Jakob M. Hebert, Brad J. Schmier, Discovery and properties of a monoclonal antibody targeting 8-oxoA, an oxidized adenine lesion in DNA and RNA, Redox Biology 62 (2023) 102658, PMC10074937
Indirect Elisa Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.