Anti-Bcl-3 Antibody (11067)
Anti-Bcl-3 Antibody (11067)
Product No.: 11067
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Clone 150-3.5 Target Bcl-3 Formats AvailableView All Product Type Monoclonal Alternate Names BCL-3, Proto-oncogene BCL3 Isotype Hamster IgG1 Applications ELISA , WB |
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse Host Species Hamster Immunogen Recombinant mouse Bcl-3- 6His. Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. State of Matter Liquid Product Preparation Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 2ug/ml. A band of approx. 64kDa is detected
End user should determine optimal concentration for each sample. Positive control: Jurkat T cell lysate.See Bassetti MFJ et al. 2009 International Immunology 21: 339-348. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human and mouse Bcl-3. Background The gene encoding Bcl-3 was first identified as the gene altered in some cases of chronic B cell lymphocytic leukemia and was later found to belong to the IkappaB subfamily of inhibitors of NFkappaB transcription factors. Bcl- 3 does not have DNA-binding activity, but it interacts with NFkappaB1 and NFkappaB2 which, when bound to DNA, repress gene transcription. Therefore, Bcl-3 may promote gene expression that is repressed by members of the NFkappaB family. Bcl-3 RNA is highly expressed in spleen and liver with lower levels of expression in heart, skeletal muscle, lung, kidney, and testes. Function Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity). {PubMed:8453667}. NCBI Gene Bank ID UniProt.org Research Area Apoptosis References & CitationsTechnical Protocols  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
