Anti-c-Jun (Phospho-Ser243) Antibody (43025)
Anti-c-Jun (Phospho-Ser243) Antibody (43025)
Product No.: 43025
Data
Immunoblot: Detection of c-Jun (phospho-Ser243) in extracts of
sorbitol-treated 293 cells, serum-treated C2C12 cells,
and UV-treated HepG2 cells or in untreated extracts.
Immunohistochemistry: Detection of c-Jun (phospho-Ser243) in paraffin-embedded human breast carcinoma tissue. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of Serine 243 (P-L-S(p)-P-I) derived from human c-Jun and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to c-Jun (Phospho-Ser243) Background c-Jun in combination with c-Fos, forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the c-jun gene. It is activated through double phosphorylation by the JNK pathway but has also a phosphorylation-independent function Antigen DetailsResearch Area Phosphospecific Antibodies References & CitationsTechnical Protocols  |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
