Anti-c-Jun (Phospho-Thr93) Antibody (43022)

Anti-c-Jun (Phospho-Thr93) Antibody (43022)

Product No.: 43022

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Target
c-Jun (Phospho-Thr93)
Product Type
Polyclonal
Isotype
Rabbit
Applications
WB,IHC

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Data

Immunohistochemistry: Detection of c-Jun (phospho-Thr93) in paraffin-embedded human breast carcinoma tissue.
Immunoblot: Detection of c-Jun (phospho-Thr93) in extracts of HeLa cells untreated or treated with PMA.
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Antibody Details

Product Details

Reactivity Species
Human
Mouse
Rat
Host Species
Rabbit
Immunogen
Peptide sequence that includes phosphorylation site of threonine 93 (T-T-T(p)-P-T) derived from human c-Jun and conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
State of Matter
Liquid
Product Preparation
Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Storage and Handling
This antibody is stable for at least one (1) year at -20°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to c-Jun (Phospho-Thr93)
Background
c-Jun in combination with c-Fos, forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the c-jun gene. It is activated through double phosphorylation by the JNK pathway but has also a phosphorylation-independent function

Antigen Details

Research Area
Phosphospecific Antibodies

References & Citations

WB,IHC
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.