Anti-Cavß Ca+2 Channel [Clone S8b-1]
Anti-Cavß Ca+2 Channel [Clone S8b-1]
Product No.: 11524
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav beta 2 Monoclonal Antibody, Clone S8B-1 (11524). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav beta 2 Monoclonal Antibody (11524) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (11524). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (11524) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.
Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (11524). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (11524) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.
Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (11524). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (11524) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Synthetic peptide corresponding to aa 189-205 of rat Cavb2 (RSPKPSANSVTSPHSKE) (accession no. NP_446303). Product Concentration Lot Specific Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-10ug/mL. A band of ~78kDa is detected.
These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human, mouse, and rat Cavb2. It does not cross-react with Cav b1, Cav b3, or Cav b4. Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Cavbeta subunits are involved in the transport of the pore-forming alpha1 subunit to the plasma membrane. They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the pore- forming subunit to the target membrane. Antigen DetailsFunction The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. {PubMed:11604404, PubMed:12042350, PubMed:1370480}. NCBI Gene Bank ID UniProt.org Research Area Ion Channels References & CitationsTechnical ProtocolsICC IF    AM |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
