Anti-Cav3.2 Ca+2 Channel [Clone S55-10]
Anti-Cav3.2 Ca+2 Channel [Clone S55-10]
Product No.: 11505
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav3.2 Monoclonal Antibody, Clone N55/10 (11505). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav3.2 Monoclonal Antibody (11505) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.2 Antibody (D) Composite.
Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody, Clone N55/10 (11505). Tissue: frozen brain section. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody (11505) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody, Clone N55/10 (11505). Tissue: hippocampus. Species: Human. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody (11505) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Fusion protein corresponding to aa 1019-1293 (II-III loop) Product Concentration Lot Specific Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-10ug/mL. A band of >260kDa is detected.Immunohistochemistry and and
Immunocytochemistry: use at 0.1-1ug/mL. Immunofluorescence: use at 1-10ug/mL. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain membranes. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human, mouse, and rat Cav3.2. It does not cross-react with Cav1.3. Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Cav3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest that certain mutations in this gene lead to childhood absence epilepsy. It has also been suggested that up-regulation of Cav3.2 may be involved in the progression of prostate cancer toward an androgen -independent stage. Antigen DetailsFunction Voltage-sensitive calcium channel that gives rise to T-type calcium currents. T-type calcium channels belong to the 'low-voltage activated (LVA)' group. A particularity of this type of channel is an opening at quite negative potentials, and a voltage-dependent inactivation (PubMed:9670923, PubMed:9930755, PubMed:27149520). T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle (Probable). They may also be involved in the modulation of firing patterns of neurons (PubMed:15048902). In the adrenal zona glomerulosa, participates in the signaling pathway leading to aldosterone production in response to either AGT/angiotensin II, or hyperkalemia (PubMed:25907736, PubMed:27729216). {PubMed:24277868, PubMed:25907736, PubMed:27149520, PubMed:27729216, PubMed:9670923, PubMed:9930755, PubMed:15048902}. NCBI Gene Bank ID UniProt.org Research Area Ion Channels References & CitationsTechnical ProtocolsICC IF    AM |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
