Anti-DNMT1 Antibody (12595)
Anti-DNMT1 Antibody (12595)
Product No.: 12595
- -
- -
Clone 4G11-C7 Target DNMT1 Formats AvailableView All Product Type Monoclonal Alternate Names AIM, CXXC9, CXXC9CXXC finger protein 9, CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNMT Isotype Mouse IgG2b Applications ELISA , WB |
- -
- -
Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse ⋅ Zebrafish Host Species Mouse Immunogen Synthetic peptide corresponding to aa 637-650 of human DNMT1 (accession no. NP_001370). Product Concentration Lot Specific Formulation PBS, pH 7.4, 0.09% sodium azide. Purified by Protein G affinity chromatography. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco ELISA Immunoblotting: use at 2ug/ml. A band of ~190kDa is detected.
These are recommended concentrations. End user should determine optimal concentrations for their applications. Positive controls: ES and H1299 cell lysates. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human, mouse, and Zebrafish DNMT1. Background DNA (cytosine-5)-methyltransferase 1 (DNMT1) is a key enzyme responsible for maintaining DNA methylation patterns, particularly during DNA replication and development. DNMT1 preferentially methylates hemimethylated DNA, ensuring the faithful transmission of epigenetic information across cell divisions. This activity is coordinated with chromatin remodeling processes, as DNMT1 interacts with histone deacetylases and other methyltransferases to regulate gene expression through both DNA and histone modifications. Disruption of normal DNMT1 function or methylation patterns has been associated with various developmental disorders and the pathogenesis of several cancers. Antibodies against DNMT1 serve as valuable tools for investigating epigenetic regulation, DNA methylation dynamics, and their implications in tumorigenesis and disease progression. Function Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306). {PubMed:16357870, PubMed:18413740, PubMed:18754681, PubMed:24623306}. NCBI Gene Bank ID UniProt.org Research Area DNA References & CitationsTechnical Protocols  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
