Anti-E. coli K99 pili Mouse Monoclonal Antibody – Library Pack (184101)

Bovine enteric colibacillosis is an economically important disease in neonatal calves caused by enterotoxigenic E. coli (ETEC) which adhere to intestinal cells using pili. Most ETEC isolated from cattle use K99 pili to bind to enterocytes of the small intestine. K99 pili are primarily composed of a repeating 18.2kDa major subunit which is responsible for binding to host glycoprotein receptor, N-glycolylneuraminic acid-GM3. A 7.1kb operon of eight genes on a plasmid encode the pili and other associated proteins responsible for regulation, transport, and assembly of K99 pili.

Purified E. coli K99 (B44) pili.

These antibodies recognize K99+ E. coli. They do not cross-react with K99 negative E. coli (B41), E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.

Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Anti-E. coli K99 pili Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies:

18401 - Anti-E. coli K99 pili Mouse Monoclonal Antibody - Clone K9911-201.3 - Isotype IgG1

18402 - Anti-E. coli K99 pili Mouse Monoclonal Antibody - Clone K9912-349.2 - Isotype IgG1

18403 - Anti-E. coli K99 pili Mouse Monoclonal Antibody - Clone K9912-418.2 - Isotype IgG1

18404 - Anti-E. coli K99 pili Mouse Monoclonal Antibody - Clone K9912-444.5 - Isotype IgG1

Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles.