Anti-E. coli K99 pili [Clone K9912-349.2]

Purified E. coli K99 (B44) pili

This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added.

Liquid

Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Next Day 2-8°C

These antibodies recognize K99+ E. coli. They do not cross-react with K99-negative E. coli (B41), E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.

Bovine enteric colibacillosis is an economically important disease in neonatal calves caused by enterotoxigenic E. coli (ETEC) which adhere to intestinal cells using pili. Most ETEC isolated from cattle use K99 pili to bind to enterocytes of the small intestine. K99 pili are primarily composed of a repeating 18.2kDa major subunit which is responsible for binding to host glycoprotein receptor, N-glycolylneuraminic acid-GM3. A 7.1kb operon of eight genes on a plasmid encode the pili and other associated proteins responsible for regulation, transport, and assembly of K99 pili.

Background for Clinical Matrices & Fecal Diagnostics

Developing reliable point-of-care diagnostics for Enterotoxigenic Escherichia coli (ETEC) requires antibodies capable of robust performance in highly complex biological environments. In veterinary medicine, neonatal calf diarrhea (commonly known as calf scours) presents a diagnostic challenge due to the presence of lipids, proteins, and complex microbiota in fecal matrices that can interfere with antibody-antigen binding.

The Leinco Technologies Anti-E. coli K99 Pili Monoclonal Antibody (Clone 18402) is selected for its high binding affinity and conformational stability, making it exceptionally well-suited for screening complex animal waste and environmental samples. By targeting the highly conserved K99 (F5) fimbrial antigen with high precision, this clone ensures minimal matrix interference and high signal-to-noise ratios, providing assay developers with a rugged component for field-deployable diagnostic kits.