Anti-E. coli K99 pili [Clone K9911-201.3]

Purified E. coli K99 (B44) pili

This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added.

Liquid

Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Next Day 2-8°C

These antibodies recognize K99+ E. coli. They do not cross-react with K99-negative E. coli (B41), E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.

Enterotoxigenic Escherichia coli (ETEC) relies heavily on fimbrial adhesins, primarily the K99 (F5) pilus, to colonize the intestinal tracts of neonatal livestock. The K99 pilus is a highly immunogenic, multimeric protein appendage composed of major structural subunits that bind specifically to host glycoproteins. Monoclonal antibodies targeting these distinct conformational epitopes on the K99 pilus are critical for developing highly sensitive immunoassays capable of neutralizing or detecting ETEC before severe secretory diarrhea occurs."

Matched Pair Recommendation: For researchers developing sandwich ELISAs or lateral flow assays (LFAs), this clone has been screened for compatibility with other K99-specific monoclonals. For optimal assay sensitivity, we recommend evaluating this antibody in tandem with our Clone K9912-444.5 or screening our complete Anti-E. coli K99 antibody with our Library Pack.