Anti-Estrogen Receptor-α (Phospho-Ser167) Antibody (43073)

Anti-Estrogen Receptor-α (Phospho-Ser167) Antibody (43073)

Product No.: 43073

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Target
Estrogen Receptor-a (Phospho-Ser167)
Product Type
Polyclonal
Isotype
Rabbit
Applications
WB,IHC,IF

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Data

Immunoblotting: Detection of ER-α (phosphoSer167) in extracts of MCF-7 cells (Lane 2) and with the antibody pre-incubated with blocking peptide (Lane 1).
Immunofluorescence: Detection of ER-α (phosphoSer167) in methanol-fixed MCF-7 cells.
Immunohistochemistry: Detection of ER-α (phosphoSer167) in paraffin-embedded human breast carcinoma tissue.
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Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Peptide sequence that includes phosphorylation site of Serine 167 (L-A-S(p)-T-N) derived from human estrogen receptor-a and conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
State of Matter
Liquid
Product Preparation
Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Storage and Handling
This antibody is stable for at least one (1) year at -20°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~66kDa is detected.

Immunofluorescence: use at dilution of 1:100-1:200.

Immunohistochemistry: use at dilution of 1:50-1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to Estrogen Receptor-a (Phospho-Ser167)
Background
Estrogen receptor alpha (ERalpha), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is one of two main types of estrogen receptor, a nuclear receptor that is activated by estrogen. In humans, ERalpha is encoded by the gene ESR1 (Estrogen Receptor 1). Estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability.

Antigen Details

NCBI Gene Bank ID
UniProt.org
Research Area
Phosphospecific Antibodies

References & Citations

WB,IHC,IF
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.