Anti-Estrogen Receptor-α (Phospho-Ser118) Antibody (43072)
Anti-Estrogen Receptor-α (Phospho-Ser118) Antibody (43072)
Product No.: 43072
Data
Immunohistochemistry: Detection of ER-α (phosphoSer118) in paraffin-embedded
human breast carcinoma tissue.
Immunofluorescence: Detection of ER-α (phosphoSer118) in methanol-fixed MCF-7
cells.
Immunoblotting: Detection of ER-α (phosphoSer118) in extracts of MDA cells
untreated or treated with estradiol. - -
- -
Antibody DetailsProduct DetailsReactivity Species Human Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of Serine 118 (Q-L-S(p)-P-F) derived from human estrogen receptor-a and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~66kDa is detected.
Immunofluorescence: use at dilution of 1:100-1:200. Immunohistochemistry: use at dilution of 1:50-1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Estrogen Receptor-a (Phospho-Ser118) Background Estrogen receptor alpha (ERalpha), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is one of two main types of estrogen receptor, a nuclear receptor that is activated by estrogen. In humans, ERalpha is encoded by the gene ESR1 (Estrogen Receptor 1). Estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability. Antigen DetailsNCBI Gene Bank ID UniProt.org Research Area Phosphospecific Antibodies References & CitationsTechnical ProtocolsIF   |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
